|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL2RB ORF mammalian expression plasmid, C-GFPSpark tag||RG80340-ACG|
|Rat IL2RB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80340-ACR|
|Rat IL2RB ORF mammalian expression plasmid, C-Flag tag||RG80340-CF|
|Rat IL2RB ORF mammalian expression plasmid, C-His tag||RG80340-CH|
|Rat IL2RB ORF mammalian expression plasmid, C-Myc tag||RG80340-CM|
|Rat IL2RB ORF mammalian expression plasmid, C-HA tag||RG80340-CY|
|Rat IL2RB Gene cDNA clone plasmid||RG80340-G|
|Rat IL2RB ORF mammalian expression plasmid, N-Flag tag||RG80340-NF|
|Rat IL2RB ORF mammalian expression plasmid, N-His tag||RG80340-NH|
|Rat IL2RB ORF mammalian expression plasmid, N-Myc tag||RG80340-NM|
|Rat IL2RB ORF mammalian expression plasmid, N-HA tag||RG80340-NY|
|Rat IL2RB natural ORF mammalian expression plasmid||RG80340-UT|
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Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.