|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL7 ORF mammalian expression plasmid, C-GFPSpark tag||RG80329-ACG|
|Rat IL7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80329-ACR|
|Rat IL7 ORF mammalian expression plasmid, C-Flag tag||RG80329-CF|
|Rat IL7 ORF mammalian expression plasmid, C-His tag||RG80329-CH|
|Rat IL7 ORF mammalian expression plasmid, C-Myc tag||RG80329-CM|
|Rat IL7 ORF mammalian expression plasmid, C-HA tag||RG80329-CY|
|Rat IL7 Gene cDNA clone plasmid||RG80329-G|
|Rat IL7 ORF mammalian expression plasmid, N-Flag tag||RG80329-NF|
|Rat IL7 ORF mammalian expression plasmid, N-His tag||RG80329-NH|
|Rat IL7 ORF mammalian expression plasmid, N-Myc tag||RG80329-NM|
|Rat IL7 ORF mammalian expression plasmid, N-HA tag||RG80329-NY|
|Rat IL7 natural ORF mammalian expression plasmid||RG80329-UT|
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IL7, also known as interleukin 7, is a hematopoietic growth factor which belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.