|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat PROM1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80324-ACG|
|Rat PROM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80324-ACR|
|Rat PROM1 ORF mammalian expression plasmid, C-Flag tag||RG80324-CF|
|Rat PROM1 ORF mammalian expression plasmid, C-His tag||RG80324-CH|
|Rat PROM1 ORF mammalian expression plasmid, C-Myc tag||RG80324-CM|
|Rat PROM1 ORF mammalian expression plasmid, C-HA tag||RG80324-CY|
|Rat PROM1 Gene cDNA clone plasmid||RG80324-G|
|Rat PROM1 ORF mammalian expression plasmid, N-Flag tag||RG80324-NF|
|Rat PROM1 ORF mammalian expression plasmid, N-His tag||RG80324-NH|
|Rat PROM1 ORF mammalian expression plasmid, N-Myc tag||RG80324-NM|
|Rat PROM1 ORF mammalian expression plasmid, N-HA tag||RG80324-NY|
|Rat PROM1 natural ORF mammalian expression plasmid||RG80324-UT|
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CD133, also known as PROM1 and Prominin 1, is a pentaspan transmembrane glycoprotein which belongs to the prominin family. It localizes to membrane protrusions and is often expressed on adult stem cells. CD133 is known to play a role in maintaining stem cell properties by suppressing differentiation. CD133 binds cholesterol in cholesterol-containing plasma membrane microdomains. It is proposed to play a role in apical plasma membrane organization of epithelial cells. CD133 is also involved in regulation of MAPK and Akt signaling pathways. Mutations in PROM1 gene have been shown to result in retinitis pigmentosa and Stargardt disease. PROM1 gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Expression of this gene is also associated with several types of cancer.