|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CSF2RB ORF mammalian expression plasmid, C-GFPSpark tag||RG80323-ACG|
|Rat CSF2RB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80323-ACR|
|Rat CSF2RB ORF mammalian expression plasmid, C-Flag tag||RG80323-CF|
|Rat CSF2RB ORF mammalian expression plasmid, C-His tag||RG80323-CH|
|Rat CSF2RB ORF mammalian expression plasmid, C-Myc tag||RG80323-CM|
|Rat CSF2RB ORF mammalian expression plasmid, C-HA tag||RG80323-CY|
|Rat CSF2RB Gene cDNA clone plasmid||RG80323-G|
|Rat CSF2RB ORF mammalian expression plasmid, N-Flag tag||RG80323-NF|
|Rat CSF2RB ORF mammalian expression plasmid, N-His tag||RG80323-NH|
|Rat CSF2RB ORF mammalian expression plasmid, N-Myc tag||RG80323-NM|
|Rat CSF2RB ORF mammalian expression plasmid, N-HA tag||RG80323-NY|
|Rat CSF2RB natural ORF mammalian expression plasmid||RG80323-UT|
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Colony stimulating factor 2 receptor, beta, low-affinity (CSF2RB) also known as CD131 antigen (CD131), cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta-chain, interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (IL3RB), is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this protein have been reported to be associated with protein alveolar proteinosis (PAP). CD131 belongs to the type I cytokine receptor family. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Defects in CD131/CSF2RB are the cause of pulmonary surfactant metabolism dysfunction type 5 (SMDP5). SMDP5 is a rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.