|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse WTAP ORF mammalian expression plasmid, C-GFPSpark tag||MG52198-ACG|
|Mouse WTAP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52198-ACR|
|Mouse WTAP ORF mammalian expression plasmid, N-GFPSpark tag||MG52198-ANG|
|Mouse WTAP ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52198-ANR|
|Mouse WTAP ORF mammalian expression plasmid, C-Flag tag||MG52198-CF|
|Mouse WTAP ORF mammalian expression plasmid, C-His tag||MG52198-CH|
|Mouse WTAP ORF mammalian expression plasmid, C-Myc tag||MG52198-CM|
|Mouse WTAP ORF mammalian expression plasmid, C-HA tag||MG52198-CY|
|Mouse WTAP Gene cDNA clone plasmid||MG52198-G|
|Mouse WTAP ORF mammalian expression plasmid, N-Flag tag||MG52198-NF|
|Mouse WTAP ORF mammalian expression plasmid, N-His tag||MG52198-NH|
|Mouse WTAP ORF mammalian expression plasmid, N-Myc tag||MG52198-NM|
|Mouse WTAP ORF mammalian expression plasmid, N-HA tag||MG52198-NY|
|Mouse WTAP natural ORF mammalian expression plasmid||MG52198-UT|
|Learn more about expression Vectors|
Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G(1)/S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.