|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CDH16 ORF mammalian expression plasmid, C-GFPSpark tag||RG80282-ACG|
|Rat CDH16 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80282-ACR|
|Rat CDH16 ORF mammalian expression plasmid, C-Flag tag||RG80282-CF|
|Rat CDH16 ORF mammalian expression plasmid, C-His tag||RG80282-CH|
|Rat CDH16 ORF mammalian expression plasmid, C-Myc tag||RG80282-CM|
|Rat CDH16 ORF mammalian expression plasmid, C-HA tag||RG80282-CY|
|Rat CDH16 Gene cDNA clone plasmid||RG80282-G|
|Rat CDH16 ORF mammalian expression plasmid, N-Flag tag||RG80282-NF|
|Rat CDH16 ORF mammalian expression plasmid, N-His tag||RG80282-NH|
|Rat CDH16 ORF mammalian expression plasmid, N-Myc tag||RG80282-NM|
|Rat CDH16 ORF mammalian expression plasmid, N-HA tag||RG80282-NY|
|Rat CDH16 natural ORF mammalian expression plasmid||RG80282-UT|
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KSP-Cadherin/Cadherin-16 is a member of the cadherin superfamily, calcium-dependent, membrane-associated glycoproteins. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. KSP-Cadherin/Cadherin-16 can be detected at later stages of tubulogenesis during human renal development and in the distal tubules of adult kidneys, no expression was found by immunohistochemistry or Western blot analysis in RCC tumour tissues and several RCC cell lines. However, despite the lack of protein expression, mRNA synthesis of KSP-Cadherin/Cadherin-16 could be detected by reverse transcriptase-polymerase chain reaction analysis in all RCC tissues and most of the RCC cell lines studied, although at a reduced level. The loss of KSP-Cadherin/Cadherin-16 protein was only observed in the malignant part of the tumour kidneys, whereas in the normal part of the affected kidneys KSP-Cadherin/Cadherin-16 expression was clearly detected. These results indicate a downregulation of Ksp-cadherin in RCC and suggest a role for this cell adhesion molecule in tumour suppression.