|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CDH13 ORF mammalian expression plasmid, C-GFPSpark tag||RG80280-ACG|
|Rat CDH13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80280-ACR|
|Rat CDH13 ORF mammalian expression plasmid, C-Flag tag||RG80280-CF|
|Rat CDH13 ORF mammalian expression plasmid, C-His tag||RG80280-CH|
|Rat CDH13 ORF mammalian expression plasmid, C-Myc tag||RG80280-CM|
|Rat CDH13 ORF mammalian expression plasmid, C-HA tag||RG80280-CY|
|Rat CDH13 Gene cDNA clone plasmid||RG80280-G|
|Rat CDH13 ORF mammalian expression plasmid, N-Flag tag||RG80280-NF|
|Rat CDH13 ORF mammalian expression plasmid, N-His tag||RG80280-NH|
|Rat CDH13 ORF mammalian expression plasmid, N-Myc tag||RG80280-NM|
|Rat CDH13 ORF mammalian expression plasmid, N-HA tag||RG80280-NY|
|Rat CDH13 natural ORF mammalian expression plasmid||RG80280-UT|
|Learn more about expression Vectors|
CDH13, also known as cadherin-13 and H Cadherin, is a member of the cadherin superfamily. CDH13 acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. CDH13 is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. CDH13 gene is hypermethylated in many types of cancer.