|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CDH5 ORF mammalian expression plasmid, C-GFPSpark tag||RG80276-ACG|
|Rat CDH5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80276-ACR|
|Rat CDH5 ORF mammalian expression plasmid, C-Flag tag||RG80276-CF|
|Rat CDH5 ORF mammalian expression plasmid, C-His tag||RG80276-CH|
|Rat CDH5 ORF mammalian expression plasmid, C-Myc tag||RG80276-CM|
|Rat CDH5 ORF mammalian expression plasmid, C-HA tag||RG80276-CY|
|Rat CDH5 Gene cDNA clone plasmid||RG80276-G|
|Rat CDH5 ORF mammalian expression plasmid, N-Flag tag||RG80276-NF|
|Rat CDH5 ORF mammalian expression plasmid, N-His tag||RG80276-NH|
|Rat CDH5 ORF mammalian expression plasmid, N-Myc tag||RG80276-NM|
|Rat CDH5 ORF mammalian expression plasmid, N-HA tag||RG80276-NY|
|Rat CDH5 natural ORF mammalian expression plasmid||RG80276-UT|
|Learn more about expression Vectors|
Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based diagnoses, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.