|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD36 ORF mammalian expression plasmid, C-GFPSpark tag||RG80263-ACG|
|Rat CD36 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80263-ACR|
|Rat CD36 ORF mammalian expression plasmid, C-Flag tag||RG80263-CF|
|Rat CD36 ORF mammalian expression plasmid, C-His tag||RG80263-CH|
|Rat CD36 ORF mammalian expression plasmid, C-Myc tag||RG80263-CM|
|Rat CD36 ORF mammalian expression plasmid, C-HA tag||RG80263-CY|
|Rat CD36 Gene cDNA clone plasmid||RG80263-G|
|Rat CD36 ORF mammalian expression plasmid, N-Flag tag||RG80263-NF|
|Rat CD36 ORF mammalian expression plasmid, N-His tag||RG80263-NH|
|Rat CD36 ORF mammalian expression plasmid, N-Myc tag||RG80263-NM|
|Rat CD36 ORF mammalian expression plasmid, N-HA tag||RG80263-NY|
|Rat CD36 natural ORF mammalian expression plasmid||RG80263-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 36 (CD36), also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb), is a member of the CD system as well as the class B scavenger receptor family of cell surface proteins. CD36 can be found on the surface of many cell types in vertebrate animals and it consists of 472 amino acids and is extensively glycosylated. It is an integral membrane protein primarily serving as receptors for thrombospondin and collagen and by the erythrocytes infected with the human malaria parasite. The role of CD36 as a cell surface receptor has been extended to that of a signal transduction molecule.