|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD34 ORF mammalian expression plasmid, C-GFPSpark tag||RG80262-ACG|
|Rat CD34 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80262-ACR|
|Rat CD34 ORF mammalian expression plasmid, C-Flag tag||RG80262-CF|
|Rat CD34 ORF mammalian expression plasmid, C-His tag||RG80262-CH|
|Rat CD34 ORF mammalian expression plasmid, C-Myc tag||RG80262-CM|
|Rat CD34 ORF mammalian expression plasmid, C-HA tag||RG80262-CY|
|Rat CD34 Gene cDNA clone plasmid||RG80262-G|
|Rat CD34 ORF mammalian expression plasmid, N-Flag tag||RG80262-NF|
|Rat CD34 ORF mammalian expression plasmid, N-His tag||RG80262-NH|
|Rat CD34 ORF mammalian expression plasmid, N-Myc tag||RG80262-NM|
|Rat CD34 ORF mammalian expression plasmid, N-HA tag||RG80262-NY|
|Rat CD34 natural ORF mammalian expression plasmid||RG80262-UT|
|Learn more about expression Vectors|
Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.