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Hemopexin / HPX Antibody, Mouse MAb

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Human HPX Antibody Product Information
Immunogen:Recombinant Human HPX protein (Catalog#10870-H08H)
Clone ID:4H6B4H3
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HPX / Hemopexin (rhHPX; Catalog#10870-H08H; Met 1-His 462; NP_000604.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human HPX Antibody Usage Guide
Specificity:Human HPX / Hemopexin
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:ELISA, ICC/IF, IF

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HPX. The detection limit for HPX is approximately 0.625ng /well.

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human HPX Antibody IF Application Image
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human HPX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human HPX monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Hemopexin / HPX Antibody, Mouse MAb, Immunofluorescence
Other HPX Antibody Products
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"