|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat PVRL1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80244-ACG|
|Rat PVRL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80244-ACR|
|Rat PVRL1 ORF mammalian expression plasmid, C-Flag tag||RG80244-CF|
|Rat PVRL1 ORF mammalian expression plasmid, C-His tag||RG80244-CH|
|Rat PVRL1 ORF mammalian expression plasmid, C-Myc tag||RG80244-CM|
|Rat PVRL1 ORF mammalian expression plasmid, C-HA tag||RG80244-CY|
|Rat PVRL1 Gene cDNA clone plasmid||RG80244-G|
|Rat PVRL1 ORF mammalian expression plasmid, N-Flag tag||RG80244-NF|
|Rat PVRL1 ORF mammalian expression plasmid, N-His tag||RG80244-NH|
|Rat PVRL1 ORF mammalian expression plasmid, N-Myc tag||RG80244-NM|
|Rat PVRL1 ORF mammalian expression plasmid, N-HA tag||RG80244-NY|
|Rat PVRL1 natural ORF mammalian expression plasmid||RG80244-UT|
|Learn more about expression Vectors|
Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.