|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat F11R ORF mammalian expression plasmid, C-GFPSpark tag||RG80240-ACG|
|Rat F11R ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80240-ACR|
|Rat F11R ORF mammalian expression plasmid, C-Flag tag||RG80240-CF|
|Rat F11R ORF mammalian expression plasmid, C-His tag||RG80240-CH|
|Rat F11R ORF mammalian expression plasmid, C-Myc tag||RG80240-CM|
|Rat F11R ORF mammalian expression plasmid, C-HA tag||RG80240-CY|
|Rat F11R Gene cDNA clone plasmid||RG80240-G|
|Rat F11R ORF mammalian expression plasmid, N-Flag tag||RG80240-NF|
|Rat F11R ORF mammalian expression plasmid, N-His tag||RG80240-NH|
|Rat F11R ORF mammalian expression plasmid, N-Myc tag||RG80240-NM|
|Rat F11R ORF mammalian expression plasmid, N-HA tag||RG80240-NY|
|Rat F11R natural ORF mammalian expression plasmid||RG80240-UT|
|Learn more about expression Vectors|
Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.