|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat KIRREL3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80225-ACG|
|Rat KIRREL3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80225-ACR|
|Rat KIRREL3 ORF mammalian expression plasmid, C-Flag tag||RG80225-CF|
|Rat KIRREL3 ORF mammalian expression plasmid, C-His tag||RG80225-CH|
|Rat KIRREL3 ORF mammalian expression plasmid, C-Myc tag||RG80225-CM|
|Rat KIRREL3 ORF mammalian expression plasmid, C-HA tag||RG80225-CY|
|Rat KIRREL3 Gene cDNA clone plasmid||RG80225-G|
|Rat KIRREL3 ORF mammalian expression plasmid, N-Flag tag||RG80225-NF|
|Rat KIRREL3 ORF mammalian expression plasmid, N-His tag||RG80225-NH|
|Rat KIRREL3 ORF mammalian expression plasmid, N-Myc tag||RG80225-NM|
|Rat KIRREL3 ORF mammalian expression plasmid, N-HA tag||RG80225-NY|
|Rat KIRREL3 natural ORF mammalian expression plasmid||RG80225-UT|
|Learn more about expression Vectors|
Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in fetalv and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotypes. Overall, these results suggest KIRREL3 is involved in the nucleogenesis of the PN through the control of neuronal migration inside the nucleus.