|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat COLEC10 ORF mammalian expression plasmid, C-GFPSpark tag||RG80217-ACG|
|Rat COLEC10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80217-ACR|
|Rat COLEC10 ORF mammalian expression plasmid, C-Flag tag||RG80217-CF|
|Rat COLEC10 ORF mammalian expression plasmid, C-His tag||RG80217-CH|
|Rat COLEC10 ORF mammalian expression plasmid, C-Myc tag||RG80217-CM|
|Rat COLEC10 ORF mammalian expression plasmid, C-HA tag||RG80217-CY|
|Rat COLEC10 Gene cDNA clone plasmid||RG80217-G|
|Rat COLEC10 ORF mammalian expression plasmid, N-Flag tag||RG80217-NF|
|Rat COLEC10 ORF mammalian expression plasmid, N-His tag||RG80217-NH|
|Rat COLEC10 ORF mammalian expression plasmid, N-Myc tag||RG80217-NM|
|Rat COLEC10 ORF mammalian expression plasmid, N-HA tag||RG80217-NY|
|Rat COLEC10 natural ORF mammalian expression plasmid||RG80217-UT|
|Learn more about expression Vectors|
COLEC10 is a member of the C-lectin family. Members of this family possess collagen-like sequences and carbohydrate recognition domains. The other members of this family are secreted proteins and bind to carbohydrate antigens on microorganisms facilitating their recognition and removal. This gene product is a cytosolic protein, a characteristic that suggests that it may have different biological functions than other C-lectins.