|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD93 ORF mammalian expression plasmid, C-GFPSpark tag||RG80207-ACG|
|Rat CD93 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80207-ACR|
|Rat CD93 ORF mammalian expression plasmid, C-Flag tag||RG80207-CF|
|Rat CD93 ORF mammalian expression plasmid, C-His tag||RG80207-CH|
|Rat CD93 ORF mammalian expression plasmid, C-Myc tag||RG80207-CM|
|Rat CD93 ORF mammalian expression plasmid, C-HA tag||RG80207-CY|
|Rat CD93 Gene cDNA clone plasmid||RG80207-G|
|Rat CD93 ORF mammalian expression plasmid, N-Flag tag||RG80207-NF|
|Rat CD93 ORF mammalian expression plasmid, N-His tag||RG80207-NH|
|Rat CD93 ORF mammalian expression plasmid, N-Myc tag||RG80207-NM|
|Rat CD93 ORF mammalian expression plasmid, N-HA tag||RG80207-NY|
|Rat CD93 natural ORF mammalian expression plasmid||RG80207-UT|
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CD93 or C1q receptor 1 (C1qR) is an about 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes, neutrophils, endothelial cells, and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR, a receptor expressed during early B-cell development, is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive, memory, and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre)plasmablasts/plasma cells, including long-lived plasma cells that showed decreased cell cycle activity, high levels of isotype-switched Ig secretion, and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches.