|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL1R2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80196-ACG|
|Rat IL1R2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80196-ACR|
|Rat IL1R2 ORF mammalian expression plasmid, C-Flag tag||RG80196-CF|
|Rat IL1R2 ORF mammalian expression plasmid, C-His tag||RG80196-CH|
|Rat IL1R2 ORF mammalian expression plasmid, C-Myc tag||RG80196-CM|
|Rat IL1R2 ORF mammalian expression plasmid, C-HA tag||RG80196-CY|
|Rat IL1R2 Gene cDNA clone plasmid||RG80196-G|
|Rat IL1R2 ORF mammalian expression plasmid, N-Flag tag||RG80196-NF|
|Rat IL1R2 ORF mammalian expression plasmid, N-His tag||RG80196-NH|
|Rat IL1R2 ORF mammalian expression plasmid, N-Myc tag||RG80196-NM|
|Rat IL1R2 ORF mammalian expression plasmid, N-HA tag||RG80196-NY|
|Rat IL1R2 natural ORF mammalian expression plasmid||RG80196-UT|
|Learn more about expression Vectors|
Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain, is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.