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14-3-3 eta / YWHAH Antibody, Mouse MAb

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human YWHAH Antibody Product Information
Immunogen:Recombinant Human 14-3-3 eta / YWHAH protein (Catalog#10847-H09E)
Clone ID:06
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Catalog#10847-H09E; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human YWHAH Antibody Usage Guide
Specificity:Human 14-3-3 eta / YWHAH
No cross-reactivity in ELISA with
E.coli cell lysate
Application:WB, ELISA, IP

WB: 0.5-2 μg/mL

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human YWHAH. The detection limit for Human YWHAH is approximately 0.078 ng/well.

IP: 2-8 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other YWHAH Antibody Products
Human YWHAH References
  • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
  • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
  • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
  • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"