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Human 14-3-3 tau / 14-3-3 theta / YWHAQ Protein (GST Tag)

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YWHAQProtein Product Information
Synonym:GW128, HS1, KCIP-1, YWHAB
Protein Construction:A DNA sequence encoding the human YWHAQ (P27348) (Met 1-Asn 245) was fused with the GST tag at the N-terminus.
Species:Human
Expressed Host:E. coli
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
YWHAQProtein QC Testing
Purity:> 88 % as determined by SDS-PAGE
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human YWHAQ/GST chimera consists of 476 amino acids and has a predicted molecular mass of 54.6 kDa. It migrates as an approxiamtely 53 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 0.15m NaCl, 20mM GSH, pH 7.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
YWHAQProtein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
10845-H09E
References
  • Cheng Y, et al. (2010) Effect of 14-3-3 tau protein on differentiation in BeWo choriocarcinoma cells. Placenta. 31(1): 60-6.
  • Umahara T, et al. (2010) 14-3-3 proteins and spinocerebellar ataxia type 1: from molecular interaction to human neuropathology. Cerebellum. 9(2): 183-9.
  • Wang B, et al. (2004) A role for 14-3-3 tau in E2F1 stabilization and DNA damage-induced apoptosis. J Biol Chem. 279(52): 54140-52.
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    • Human 14-3-3 tau / 14-3-3 theta / YWHAQ Protein (GST Tag) SDS-PAGE
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