|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat TNFRSF9 ORF mammalian expression plasmid, C-GFPSpark tag||RG80176-ACG|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80176-ACR|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-Flag tag||RG80176-CF|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-His tag||RG80176-CH|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-Myc tag||RG80176-CM|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-HA tag||RG80176-CY|
|Rat TNFRSF9 Gene cDNA clone plasmid||RG80176-G|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-Flag tag||RG80176-NF|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-His tag||RG80176-NH|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-Myc tag||RG80176-NM|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-HA tag||RG80176-NY|
|Rat TNFRSF9 natural ORF mammalian expression plasmid||RG80176-UT|
|Learn more about expression Vectors|
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.