|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IFNA1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80174-ACG|
|Rat IFNA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80174-ACR|
|Rat IFNA1 ORF mammalian expression plasmid, C-Flag tag||RG80174-CF|
|Rat IFNA1 ORF mammalian expression plasmid, C-His tag||RG80174-CH|
|Rat IFNA1 ORF mammalian expression plasmid, C-Myc tag||RG80174-CM|
|Rat IFNA1 ORF mammalian expression plasmid, C-HA tag||RG80174-CY|
|Rat IFNA1 Gene cDNA clone plasmid||RG80174-G|
|Rat IFNA1 ORF mammalian expression plasmid, N-Flag tag||RG80174-NF|
|Rat IFNA1 ORF mammalian expression plasmid, N-His tag||RG80174-NH|
|Rat IFNA1 ORF mammalian expression plasmid, N-Myc tag||RG80174-NM|
|Rat IFNA1 ORF mammalian expression plasmid, N-HA tag||RG80174-NY|
|Rat IFNA1 natural ORF mammalian expression plasmid||RG80174-UT|
|Learn more about expression Vectors|
IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells. They belong to the large class of glycoproteins known as cytokines. IFNs stimulate the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs can activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they also increase the ability of uninfected host cells to resist new infection by virus.Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has antiviral activities.