|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL11 ORF mammalian expression plasmid, C-GFPSpark tag||RG80169-ACG|
|Rat IL11 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80169-ACR|
|Rat IL11 ORF mammalian expression plasmid, C-Flag tag||RG80169-CF|
|Rat IL11 ORF mammalian expression plasmid, C-His tag||RG80169-CH|
|Rat IL11 ORF mammalian expression plasmid, C-Myc tag||RG80169-CM|
|Rat IL11 ORF mammalian expression plasmid, C-HA tag||RG80169-CY|
|Rat IL11 Gene cDNA clone plasmid||RG80169-G|
|Rat IL11 ORF mammalian expression plasmid, N-Flag tag||RG80169-NF|
|Rat IL11 ORF mammalian expression plasmid, N-His tag||RG80169-NH|
|Rat IL11 ORF mammalian expression plasmid, N-Myc tag||RG80169-NM|
|Rat IL11 ORF mammalian expression plasmid, N-HA tag||RG80169-NY|
|Rat IL11 natural ORF mammalian expression plasmid||RG80169-UT|
|Learn more about expression Vectors|
IL11 is a multifunctional cytokine first isolated in 1990 from bone marrow-derived stromal cells. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL11 is also known under the names adipogenesis inhibitory factor (AGIF) and oprelvekin. IL11 can improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. IL11 stimulates the growth of certain lymphocytes and, in the murine model, stimulates an increase in the cortical thickness and strength of long bones. As a signaling molecule, IL11 has a variety of functions associated with its receptor interleukin 11 receptor alpha; such functions include placentation and to some extent of decidualization.