|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat TNFSF11 ORF mammalian expression plasmid, C-GFPSpark tag||RG80165-ACG|
|Rat TNFSF11 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80165-ACR|
|Rat TNFSF11 ORF mammalian expression plasmid, C-Flag tag||RG80165-CF|
|Rat TNFSF11 ORF mammalian expression plasmid, C-His tag||RG80165-CH|
|Rat TNFSF11 ORF mammalian expression plasmid, C-Myc tag||RG80165-CM|
|Rat TNFSF11 ORF mammalian expression plasmid, C-HA tag||RG80165-CY|
|Rat TNFSF11 Gene cDNA clone plasmid||RG80165-G|
|Rat TNFSF11 ORF mammalian expression plasmid, N-Flag tag||RG80165-NF|
|Rat TNFSF11 ORF mammalian expression plasmid, N-His tag||RG80165-NH|
|Rat TNFSF11 ORF mammalian expression plasmid, N-Myc tag||RG80165-NM|
|Rat TNFSF11 ORF mammalian expression plasmid, N-HA tag||RG80165-NY|
|Rat TNFSF11 natural ORF mammalian expression plasmid||RG80165-UT|
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Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.