|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat TNFSF8 ORF mammalian expression plasmid, C-GFPSpark tag||RG80148-ACG|
|Rat TNFSF8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80148-ACR|
|Rat TNFSF8 ORF mammalian expression plasmid, C-Flag tag||RG80148-CF|
|Rat TNFSF8 ORF mammalian expression plasmid, C-His tag||RG80148-CH|
|Rat TNFSF8 ORF mammalian expression plasmid, C-Myc tag||RG80148-CM|
|Rat TNFSF8 ORF mammalian expression plasmid, C-HA tag||RG80148-CY|
|Rat TNFSF8 Gene cDNA clone plasmid||RG80148-G|
|Rat TNFSF8 ORF mammalian expression plasmid, N-Flag tag||RG80148-NF|
|Rat TNFSF8 ORF mammalian expression plasmid, N-His tag||RG80148-NH|
|Rat TNFSF8 ORF mammalian expression plasmid, N-Myc tag||RG80148-NM|
|Rat TNFSF8 ORF mammalian expression plasmid, N-HA tag||RG80148-NY|
|Rat TNFSF8 natural ORF mammalian expression plasmid||RG80148-UT|
|Learn more about expression Vectors|
CD30 ligand (CD30L), also known as CD153 and TNFSF8, is a membrane-associated glycoprotein belonging to the TNF superfamily and TNFR superfamily, and is a specific ligand for CD30/TNFRSF8 originally described as a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. CD30L is a type-II membrane glycoprotein expressed on activated T cells, stimulated monocyte-macrophages, granulocytes, eosinophils, and some Burkitt-like lymphoma cell lines. CD30L is capable of transducing signals through CD30 on different CD30+ lymphoma cell lines, and mediates pleiotropic biologic effects including cell proliferation, activation, differentiation, as well as cell death by apoptosis. CD30-CD30 ligand interaction has been suggested to have a pathophysiologic role in malignant lymphomas, particularly Hodgkin disease, large cell anaplastic lymphomas and Burkitt lymphomas, and is also involved in activation and functioning of the T cell-dependent immune response. Thus, CD153 and its receptor CD30 are regarded as therapeutic targets in hematologic malignancies, autoimmune and inflammatory diseases.