|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat HFE2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80142-ACG|
|Rat HFE2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80142-ACR|
|Rat HFE2 ORF mammalian expression plasmid, C-Flag tag||RG80142-CF|
|Rat HFE2 ORF mammalian expression plasmid, C-His tag||RG80142-CH|
|Rat HFE2 ORF mammalian expression plasmid, C-Myc tag||RG80142-CM|
|Rat HFE2 ORF mammalian expression plasmid, C-HA tag||RG80142-CY|
|Rat HFE2 Gene cDNA clone plasmid||RG80142-G|
|Rat HFE2 ORF mammalian expression plasmid, N-Flag tag||RG80142-NF|
|Rat HFE2 ORF mammalian expression plasmid, N-His tag||RG80142-NH|
|Rat HFE2 ORF mammalian expression plasmid, N-Myc tag||RG80142-NM|
|Rat HFE2 ORF mammalian expression plasmid, N-HA tag||RG80142-NY|
|Rat HFE2 natural ORF mammalian expression plasmid||RG80142-UT|
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Hemojuvelin, also known as HFE2, is a membrane-bound and soluble protein which belongs to the repulsive guidance molecule (RGM) family. It is known that RGMs function through Neogenin, a homologue of the Netrin receptor deleted in colon cancer. In mammals, RGM family consists of three glycoproteins which have discrete expression patterns and functions (RGM-A, RGM-B, and RGM-C). Hemojuvelin is expressed in adult and fetal liver, heart, and skeletal muscle. Hemojuvelin acts as a bone morphogenetic protein (BMP) coreceptor. Enhancement of BMP signaling regulates hepcidin (HAMP) expression and iron metabolism. It plays a key role in iron metabolism. Hemojuvelin represents the cellular receptor for hepcidin. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. Defects in hemojuvelin are the cause of hemochromatosis type 2A, also known as juvenile hemochromatosis (JH).