|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat TDGF1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80135-ACG|
|Rat TDGF1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80135-ACR|
|Rat TDGF1 ORF mammalian expression plasmid, C-Flag tag||RG80135-CF|
|Rat TDGF1 ORF mammalian expression plasmid, C-His tag||RG80135-CH|
|Rat TDGF1 ORF mammalian expression plasmid, C-Myc tag||RG80135-CM|
|Rat TDGF1 ORF mammalian expression plasmid, C-HA tag||RG80135-CY|
|Rat TDGF1 Gene cDNA clone plasmid||RG80135-G|
|Rat TDGF1 ORF mammalian expression plasmid, N-Flag tag||RG80135-NF|
|Rat TDGF1 ORF mammalian expression plasmid, N-His tag||RG80135-NH|
|Rat TDGF1 ORF mammalian expression plasmid, N-Myc tag||RG80135-NM|
|Rat TDGF1 ORF mammalian expression plasmid, N-HA tag||RG80135-NY|
|Rat TDGF1 natural ORF mammalian expression plasmid||RG80135-UT|
|Learn more about expression Vectors|
Cripto/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. Before gastrulation, Cripto is asymmetrically expressed in a proximal–distal gradient in the epiblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal–distal asymmetry into an orthogonal anterior–posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis, activation of c-Jun-NH(2)-terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus, Cripto is a unique target, and Immunohistochemistry to Cripto could be of therapeutic value for human cancers.