|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat ERBB4 ORF mammalian expression plasmid, C-GFPSpark tag||RG80125-ACG|
|Rat ERBB4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80125-ACR|
|Rat ERBB4 ORF mammalian expression plasmid, C-Flag tag||RG80125-CF|
|Rat ERBB4 ORF mammalian expression plasmid, C-His tag||RG80125-CH|
|Rat ERBB4 ORF mammalian expression plasmid, C-Myc tag||RG80125-CM|
|Rat ERBB4 ORF mammalian expression plasmid, C-HA tag||RG80125-CY|
|Rat ERBB4 Gene cDNA clone plasmid||RG80125-G|
|Rat ERBB4 ORF mammalian expression plasmid, N-Flag tag||RG80125-NF|
|Rat ERBB4 ORF mammalian expression plasmid, N-His tag||RG80125-NH|
|Rat ERBB4 ORF mammalian expression plasmid, N-Myc tag||RG80125-NM|
|Rat ERBB4 ORF mammalian expression plasmid, N-HA tag||RG80125-NY|
|Rat ERBB4 natural ORF mammalian expression plasmid||RG80125-UT|
|Learn more about expression Vectors|
ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.