|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat TGFB1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80116-ACG|
|Rat TGFB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80116-ACR|
|Rat TGFB1 ORF mammalian expression plasmid, C-Flag tag||RG80116-CF|
|Rat TGFB1 ORF mammalian expression plasmid, C-His tag||RG80116-CH|
|Rat TGFB1 ORF mammalian expression plasmid, C-Myc tag||RG80116-CM|
|Rat TGFB1 ORF mammalian expression plasmid, C-HA tag||RG80116-CY|
|Rat TGFB1 Gene cDNA clone plasmid||RG80116-G|
|Rat TGFB1 ORF mammalian expression plasmid, N-Flag tag||RG80116-NF|
|Rat TGFB1 ORF mammalian expression plasmid, N-His tag||RG80116-NH|
|Rat TGFB1 ORF mammalian expression plasmid, N-Myc tag||RG80116-NM|
|Rat TGFB1 ORF mammalian expression plasmid, N-HA tag||RG80116-NY|
|Rat TGFB1 natural ORF mammalian expression plasmid||RG80116-UT|
|Learn more about expression Vectors|
TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.