|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat ERBB3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80111-ACG|
|Rat ERBB3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80111-ACR|
|Rat ERBB3 ORF mammalian expression plasmid, C-Flag tag||RG80111-CF|
|Rat ERBB3 ORF mammalian expression plasmid, C-His tag||RG80111-CH|
|Rat ERBB3 ORF mammalian expression plasmid, C-Myc tag||RG80111-CM|
|Rat ERBB3 ORF mammalian expression plasmid, C-HA tag||RG80111-CY|
|Rat ERBB3 Gene cDNA clone plasmid||RG80111-G|
|Rat ERBB3 ORF mammalian expression plasmid, N-Flag tag||RG80111-NF|
|Rat ERBB3 ORF mammalian expression plasmid, N-His tag||RG80111-NH|
|Rat ERBB3 ORF mammalian expression plasmid, N-Myc tag||RG80111-NM|
|Rat ERBB3 ORF mammalian expression plasmid, N-HA tag||RG80111-NY|
|Rat ERBB3 natural ORF mammalian expression plasmid||RG80111-UT|
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ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.