|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat EFNA2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80110-ACG|
|Rat EFNA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80110-ACR|
|Rat EFNA2 ORF mammalian expression plasmid, C-Flag tag||RG80110-CF|
|Rat EFNA2 ORF mammalian expression plasmid, C-His tag||RG80110-CH|
|Rat EFNA2 ORF mammalian expression plasmid, C-Myc tag||RG80110-CM|
|Rat EFNA2 ORF mammalian expression plasmid, C-HA tag||RG80110-CY|
|Rat EFNA2 Gene cDNA clone plasmid||RG80110-G|
|Rat EFNA2 ORF mammalian expression plasmid, N-Flag tag||RG80110-NF|
|Rat EFNA2 ORF mammalian expression plasmid, N-His tag||RG80110-NH|
|Rat EFNA2 ORF mammalian expression plasmid, N-Myc tag||RG80110-NM|
|Rat EFNA2 ORF mammalian expression plasmid, N-HA tag||RG80110-NY|
|Rat EFNA2 natural ORF mammalian expression plasmid||RG80110-UT|
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Ephrin-A2 also known as EFNA2 or EPH-related receptor tyrosine kinase ligand 6, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A2 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A2 regulates the position-specific affinity of limb mesenchyme and is involved in cartilage pattern formation in the limb.