|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat FIGF ORF mammalian expression plasmid, C-GFPSpark tag||RG80104-ACG|
|Rat FIGF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80104-ACR|
|Rat FIGF ORF mammalian expression plasmid, C-Flag tag||RG80104-CF|
|Rat FIGF ORF mammalian expression plasmid, C-His tag||RG80104-CH|
|Rat FIGF ORF mammalian expression plasmid, C-Myc tag||RG80104-CM|
|Rat FIGF ORF mammalian expression plasmid, C-HA tag||RG80104-CY|
|Rat FIGF Gene cDNA clone plasmid||RG80104-G|
|Rat FIGF ORF mammalian expression plasmid, N-Flag tag||RG80104-NF|
|Rat FIGF ORF mammalian expression plasmid, N-His tag||RG80104-NH|
|Rat FIGF ORF mammalian expression plasmid, N-Myc tag||RG80104-NM|
|Rat FIGF ORF mammalian expression plasmid, N-HA tag||RG80104-NY|
|Rat FIGF natural ORF mammalian expression plasmid||RG80104-UT|
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Vascular endothelial growth factor D (VEGF-D), also known as C-fos induced growth factor (FIGF), belongs to the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. FIGF protein is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. FIGF protein is secreted as a non-covelent homodimer in an antiparallel fashion. Human FIGF protein is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. FIGF protein is structurally and functionally similar to VEGF-C. Therefore, FIGF protein binds and activates VEGFR-2 (Flk1) and VEGFR-3 (Flt4) receptors, and may particularly be involved in cancers, such as breast cancer, epithelial ovarian carcinoma and so on.