|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat PGC ORF mammalian expression plasmid, C-GFPSpark tag||RG80097-ACG|
|Rat PGC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80097-ACR|
|Rat PGC ORF mammalian expression plasmid, C-Flag tag||RG80097-CF|
|Rat PGC ORF mammalian expression plasmid, C-His tag||RG80097-CH|
|Rat PGC ORF mammalian expression plasmid, C-Myc tag||RG80097-CM|
|Rat PGC ORF mammalian expression plasmid, C-HA tag||RG80097-CY|
|Rat PGC Gene cDNA clone plasmid||RG80097-G|
|Rat PGC ORF mammalian expression plasmid, N-Flag tag||RG80097-NF|
|Rat PGC ORF mammalian expression plasmid, N-His tag||RG80097-NH|
|Rat PGC ORF mammalian expression plasmid, N-Myc tag||RG80097-NM|
|Rat PGC ORF mammalian expression plasmid, N-HA tag||RG80097-NY|
|Rat PGC natural ORF mammalian expression plasmid||RG80097-UT|
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Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. At low PH conditions, Pepsinogen C undergoes conversion into active enzyme. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.