|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CA13 ORF mammalian expression plasmid, C-GFPSpark tag||RG80088-ACG|
|Rat CA13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80088-ACR|
|Rat CA13 ORF mammalian expression plasmid, N-GFPSpark tag||RG80088-ANG|
|Rat CA13 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80088-ANR|
|Rat CA13 ORF mammalian expression plasmid, C-Flag tag||RG80088-CF|
|Rat CA13 ORF mammalian expression plasmid, C-His tag||RG80088-CH|
|Rat CA13 ORF mammalian expression plasmid, C-Myc tag||RG80088-CM|
|Rat CA13 ORF mammalian expression plasmid, C-HA tag||RG80088-CY|
|Rat CA13 Gene cDNA clone plasmid||RG80088-G|
|Rat CA13 ORF mammalian expression plasmid, N-Flag tag||RG80088-NF|
|Rat CA13 ORF mammalian expression plasmid, N-His tag||RG80088-NH|
|Rat CA13 ORF mammalian expression plasmid, N-Myc tag||RG80088-NM|
|Rat CA13 ORF mammalian expression plasmid, N-HA tag||RG80088-NY|
|Rat CA13 natural ORF mammalian expression plasmid||RG80088-UT|
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The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.