|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat ERBB2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80079-ACG|
|Rat ERBB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80079-ACR|
|Rat ERBB2 ORF mammalian expression plasmid, C-Flag tag||RG80079-CF|
|Rat ERBB2 ORF mammalian expression plasmid, C-His tag||RG80079-CH|
|Rat ERBB2 ORF mammalian expression plasmid, C-Myc tag||RG80079-CM|
|Rat ERBB2 ORF mammalian expression plasmid, C-HA tag||RG80079-CY|
|Rat ERBB2 Gene cDNA clone plasmid||RG80079-M|
|Rat ERBB2 ORF mammalian expression plasmid, N-Flag tag||RG80079-NF|
|Rat ERBB2 ORF mammalian expression plasmid, N-His tag||RG80079-NH|
|Rat ERBB2 ORF mammalian expression plasmid, N-Myc tag||RG80079-NM|
|Rat ERBB2 ORF mammalian expression plasmid, N-HA tag||RG80079-NY|
|Rat ERBB2 natural ORF mammalian expression plasmid||RG80079-UT|
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Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.