|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CXCL10 ORF mammalian expression plasmid, C-GFPSpark tag||RG80057-ACG|
|Rat CXCL10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80057-ACR|
|Rat CXCL10 ORF mammalian expression plasmid, C-Flag tag||RG80057-CF|
|Rat CXCL10 ORF mammalian expression plasmid, C-His tag||RG80057-CH|
|Rat CXCL10 ORF mammalian expression plasmid, C-Myc tag||RG80057-CM|
|Rat CXCL10 ORF mammalian expression plasmid, C-HA tag||RG80057-CY|
|Rat CXCL10 Gene cDNA clone plasmid||RG80057-M|
|Rat CXCL10 ORF mammalian expression plasmid, N-Flag tag||RG80057-NF|
|Rat CXCL10 ORF mammalian expression plasmid, N-His tag||RG80057-NH|
|Rat CXCL10 ORF mammalian expression plasmid, N-Myc tag||RG80057-NM|
|Rat CXCL10 ORF mammalian expression plasmid, N-HA tag||RG80057-NY|
|Rat CXCL10 natural ORF mammalian expression plasmid||RG80057-UT|
|Learn more about expression Vectors|
CXCL10, also known as crg-2, is a chemokine of the CXC subfamily and ligand for the receptor CXCR3. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. CXCL10 has a three-dimensional crystal structure. Its signaling is mediated by the g protein-coupled receptor CXCR3, which is expressed on activated T cells and plays an important role in directing the migration of T cells, especially during Th1 responses. Binding of CXCL10 to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. It is chemotactic for monocytes and T-lymphocytes. CXCL10 can be secreted by several cell types in response to IFN-γ. Baseline pre-treatment plasma levels of CXCL10 are elevated in patients chronically infected with hepatitis C virus (HCV) of genotypes 1 or 4 who do not achieve a sustained viral response (SVR) after completion of antiviral therapy.