|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat EPO ORF mammalian expression plasmid, C-GFPSpark tag||RG80055-ACG|
|Rat EPO ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80055-ACR|
|Rat EPO ORF mammalian expression plasmid, C-Flag tag||RG80055-CF|
|Rat EPO ORF mammalian expression plasmid, C-His tag||RG80055-CH|
|Rat EPO ORF mammalian expression plasmid, C-Myc tag||RG80055-CM|
|Rat EPO ORF mammalian expression plasmid, C-HA tag||RG80055-CY|
|Rat EPO Gene cDNA clone plasmid||RG80055-M|
|Rat EPO ORF mammalian expression plasmid, N-Flag tag||RG80055-NF|
|Rat EPO ORF mammalian expression plasmid, N-His tag||RG80055-NH|
|Rat EPO ORF mammalian expression plasmid, N-Myc tag||RG80055-NM|
|Rat EPO ORF mammalian expression plasmid, N-HA tag||RG80055-NY|
|Rat EPO natural ORF mammalian expression plasmid||RG80055-UT|
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Erythropoietin is a member of the EPO / TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. It has a longer circulating half-life in vivo. Erythropoietin is being much misused as a performance-enhancing drug in endurance athletes.