|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CCL3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80044-ACG|
|Rat CCL3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80044-ACR|
|Rat CCL3 ORF mammalian expression plasmid, C-Flag tag||RG80044-CF|
|Rat CCL3 ORF mammalian expression plasmid, C-His tag||RG80044-CH|
|Rat CCL3 ORF mammalian expression plasmid, C-Myc tag||RG80044-CM|
|Rat CCL3 ORF mammalian expression plasmid, C-HA tag||RG80044-CY|
|Rat CCL3 Gene cDNA clone plasmid||RG80044-M|
|Rat CCL3 ORF mammalian expression plasmid, N-Flag tag||RG80044-NF|
|Rat CCL3 ORF mammalian expression plasmid, N-His tag||RG80044-NH|
|Rat CCL3 ORF mammalian expression plasmid, N-Myc tag||RG80044-NM|
|Rat CCL3 ORF mammalian expression plasmid, N-HA tag||RG80044-NY|
|Rat CCL3 natural ORF mammalian expression plasmid||RG80044-UT|
|Learn more about expression Vectors|
CCL3 is a cytokine belonging to the CC chemokine family. Chemokines are a family of structurally related leukocyte chemoattractant cytokines that play a central role during immunoregulatory and inflammation processes. All chemokines contain four conserved cysteines linked by disulfide bonds, and two major subfamilies, namely CXC and CC, are defined on the basis of the first two cysteines which are separated by one amino acid or are adjacent. CCL3 is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes.