|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CXCL1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80039-ACG|
|Rat CXCL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80039-ACR|
|Rat CXCL1 ORF mammalian expression plasmid, C-Flag tag||RG80039-CF|
|Rat CXCL1 ORF mammalian expression plasmid, C-His tag||RG80039-CH|
|Rat CXCL1 ORF mammalian expression plasmid, C-Myc tag||RG80039-CM|
|Rat CXCL1 ORF mammalian expression plasmid, C-HA tag||RG80039-CY|
|Rat CXCL1 Gene cDNA clone plasmid||RG80039-M|
|Rat CXCL1 ORF mammalian expression plasmid, N-Flag tag||RG80039-NF|
|Rat CXCL1 ORF mammalian expression plasmid, N-His tag||RG80039-NH|
|Rat CXCL1 ORF mammalian expression plasmid, N-Myc tag||RG80039-NM|
|Rat CXCL1 ORF mammalian expression plasmid, N-HA tag||RG80039-NY|
|Rat CXCL1 natural ORF mammalian expression plasmid||RG80039-UT|
|Learn more about expression Vectors|
The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.