|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat MSTN ORF mammalian expression plasmid, C-GFPSpark tag||RG80032-ACG|
|Rat MSTN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80032-ACR|
|Rat MSTN ORF mammalian expression plasmid, C-Flag tag||RG80032-CF|
|Rat MSTN ORF mammalian expression plasmid, C-His tag||RG80032-CH|
|Rat MSTN ORF mammalian expression plasmid, C-Myc tag||RG80032-CM|
|Rat MSTN ORF mammalian expression plasmid, C-HA tag||RG80032-CY|
|Rat MSTN Gene cDNA clone plasmid||RG80032-M|
|Rat MSTN ORF mammalian expression plasmid, N-Flag tag||RG80032-NF|
|Rat MSTN ORF mammalian expression plasmid, N-His tag||RG80032-NH|
|Rat MSTN ORF mammalian expression plasmid, N-Myc tag||RG80032-NM|
|Rat MSTN ORF mammalian expression plasmid, N-HA tag||RG80032-NY|
|Rat MSTN natural ORF mammalian expression plasmid||RG80032-UT|
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GDF-8 / Myostatin / MSTN is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF-8 / Myostatin / MSTN is highly expressed in skeletal muscle, and myostatin loss-of-function leads to doubling of skeletal muscle mass. Experiments in mice have improved that GDF-8 / Myostatin / MSTN is a key regulator of mesenchymal stem cell proliferation and differentiation, and mice lacking Myostatin encoding gene show decreased body fat and a generalized increase in bone density and strength. The increase in bone density is observed in most anatomical regions, including the limbs, spine, and jaw, and myostatin inhibitors have been observed to significantly increase bone formation. GDF-8 / Myostatin / MSTN is also expressed in the early phases of fracture healing, and GDF-8 / Myostatin / MSTN deficiency leads to increased fracture callus size and strength. Together, these data suggest that GDF-8 / Myostatin / MSTN has direct effects on the proliferation and differentiation of osteoprogenitor cells, and that GDF-8/Myostatin/MSTN antagonists and inhibitors are likely to enhance both muscle mass and bone strength.