|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat GHR ORF mammalian expression plasmid, C-GFPSpark tag||RG80029-ACG|
|Rat GHR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80029-ACR|
|Rat GHR ORF mammalian expression plasmid, C-Flag tag||RG80029-CF|
|Rat GHR ORF mammalian expression plasmid, C-His tag||RG80029-CH|
|Rat GHR ORF mammalian expression plasmid, C-Myc tag||RG80029-CM|
|Rat GHR ORF mammalian expression plasmid, C-HA tag||RG80029-CY|
|Rat GHR Gene cDNA clone plasmid||RG80029-M|
|Rat GHR ORF mammalian expression plasmid, N-Flag tag||RG80029-NF|
|Rat GHR ORF mammalian expression plasmid, N-His tag||RG80029-NH|
|Rat GHR ORF mammalian expression plasmid, N-Myc tag||RG80029-NM|
|Rat GHR ORF mammalian expression plasmid, N-HA tag||RG80029-NY|
|Rat GHR natural ORF mammalian expression plasmid||RG80029-UT|
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Growth hormone receptor, also known as GH receptor and GHR, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and type 1 subfamily. GHR contains one fibronectin type-III domain. Growth hormone receptor / GHR is expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 of GHR is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression of GHR in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 of GHR is expressed in lung, stomach and muscle. Growth hormone receptor / GHR is a receptor for pituitary gland growth hormone. It is involved in regulating postnatal body growth. On ligand binding, it couples to the JAK2 / STAT5 pathway. Isoform 2 of GHR up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Defects in GHR are a cause of Laron syndrome (LARS) which is a severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may also be a cause of idiopathic short stature autosomal (ISSA) which is defined by a subnormal rate of growth.