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恒河猴 AMY2B 基因全长ORF克隆

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Cynomolgus AMY2B cDNA Clone Product Information
NCBI RefSeq:NM_001266186.1
RefSeq ORF Size:1536bp
cDNA Description:Full length Clone DNA of Macaca mulatta (Rhesus monkey) amylase, alpha 2B (pancreatic).
Gene Synonym:AMY2A
Species:Rhesus
Vector:pUC19 Vector
Plasmid:pUC19-cynoAMY2B
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 121C/A(L41I), 673A/G(I225V); 111T/G, 150G/A, 162G/A, 903C/T, 1269C/T, 1413T/C not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Cynomolgus AMY2B Gene Plasmid Map
Cynomolgus monkey AMY2B Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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Background

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase is the major form of amylase found in humans and other mammals. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human α-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.

References
  • Abe A. et al., 2005, FEBS J. 272 (23): 6145-53.
  • Aghajari N. et al., 1998, Protein Sci. 7 (3): 564-72.
  • Ramasubbu N. et al., 1996, Acta Crystallographica Section D Biological Crystallography. 52 (3): 435-46.
  • Size / Price
    Catalog: CG90526-U
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    • Cynomolgus monkey AMY2B Gene cDNA Clone (full-length ORF Clone)
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