|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD16a ORF mammalian expression plasmid, C-GFPSpark tag||RG80017-ACG|
|Rat CD16a ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80017-ACR|
|Rat CD16a ORF mammalian expression plasmid, C-Flag tag||RG80017-CF|
|Rat CD16a ORF mammalian expression plasmid, C-His tag||RG80017-CH|
|Rat CD16a ORF mammalian expression plasmid, C-Myc tag||RG80017-CM|
|Rat CD16a ORF mammalian expression plasmid, C-HA tag||RG80017-CY|
|Rat CD16a Gene cDNA clone plasmid||RG80017-M|
|Rat CD16a ORF mammalian expression plasmid, N-Flag tag||RG80017-NF|
|Rat CD16a ORF mammalian expression plasmid, N-His tag||RG80017-NH|
|Rat CD16a ORF mammalian expression plasmid, N-Myc tag||RG80017-NM|
|Rat CD16a ORF mammalian expression plasmid, N-HA tag||RG80017-NY|
|Rat CD16a natural ORF mammalian expression plasmid||RG80017-UT|
|Learn more about expression Vectors|
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.