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|Recombinant Human CD40 protein (Catalog#10774-H08H)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was obtained from a rabbit immunized with purified, recombinant Human CD40 extracellular domain (rh CD40; Catalog#10774-H08H; NP_001241.1; Met 1-Arg 193).|
|Human CD40 / TNFRSF5 / Bp50
No cross-reactivity in ELISA with
Human CD27 / TNFRSF7
Mouse CD27 / TNFRSF7
Human CD30 / TNFRSF8
Human DR6 / TNFRSF21
Human CD137 / 4-1BB
Human TNFRSF1B / CD120b
Human HVEM / TNFRSF14
Mouse HVEM / TNFRSF14
Human RELT / TNFRSF19L
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD40. The detection limit for Human CD40 is 0.0049 ng/well.
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.