|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat VEGFA ORF mammalian expression plasmid, C-GFPSpark tag||RG80006-ACG|
|Rat VEGFA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80006-ACR|
|Rat VEGFA ORF mammalian expression plasmid, C-Flag tag||RG80006-CF|
|Rat VEGFA ORF mammalian expression plasmid, C-His tag||RG80006-CH|
|Rat VEGFA ORF mammalian expression plasmid, C-Myc tag||RG80006-CM|
|Rat VEGFA ORF mammalian expression plasmid, C-HA tag||RG80006-CY|
|Rat VEGFA Gene cDNA clone plasmid||RG80006-G|
|Rat VEGFA ORF mammalian expression plasmid, N-Flag tag||RG80006-NF|
|Rat VEGFA ORF mammalian expression plasmid, N-His tag||RG80006-NH|
|Rat VEGFA ORF mammalian expression plasmid, N-Myc tag||RG80006-NM|
|Rat VEGFA ORF mammalian expression plasmid, N-HA tag||RG80006-NY|
|Rat VEGFA natural ORF mammalian expression plasmid||RG80006-UT|
|Learn more about expression Vectors|
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.