|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat MET ORF mammalian expression plasmid, C-GFPSpark tag||RG80004-ACG|
|Rat MET ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80004-ACR|
|Rat MET ORF mammalian expression plasmid, C-Flag tag||RG80004-CF|
|Rat MET ORF mammalian expression plasmid, C-His tag||RG80004-CH|
|Rat MET ORF mammalian expression plasmid, C-Myc tag||RG80004-CM|
|Rat MET ORF mammalian expression plasmid, C-HA tag||RG80004-CY|
|Rat MET Gene cDNA clone plasmid||RG80004-M|
|Rat MET ORF mammalian expression plasmid, N-Flag tag||RG80004-NF|
|Rat MET ORF mammalian expression plasmid, N-His tag||RG80004-NH|
|Rat MET ORF mammalian expression plasmid, N-Myc tag||RG80004-NM|
|Rat MET ORF mammalian expression plasmid, N-HA tag||RG80004-NY|
|Rat MET natural ORF mammalian expression plasmid||RG80004-UT|
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Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.