|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat GpT2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80002-ACG|
|Rat GpT2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80002-ACR|
|Rat GpT2 ORF mammalian expression plasmid, N-GFPSpark tag||RG80002-ANG|
|Rat GpT2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80002-ANR|
|Rat GpT2 ORF mammalian expression plasmid, C-Flag tag||RG80002-CF|
|Rat GpT2 ORF mammalian expression plasmid, C-His tag||RG80002-CH|
|Rat GpT2 ORF mammalian expression plasmid, C-Myc tag||RG80002-CM|
|Rat GpT2 ORF mammalian expression plasmid, C-HA tag||RG80002-CY|
|Rat GpT2 Gene cDNA clone plasmid||RG80002-M|
|Rat GpT2 ORF mammalian expression plasmid, N-Flag tag||RG80002-NF|
|Rat GpT2 ORF mammalian expression plasmid, N-His tag||RG80002-NH|
|Rat GpT2 ORF mammalian expression plasmid, N-Myc tag||RG80002-NM|
|Rat GpT2 ORF mammalian expression plasmid, N-HA tag||RG80002-NY|
|Rat GpT2 natural ORF mammalian expression plasmid||RG80002-UT|
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Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (Gpt), is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. Gpt1 is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level, whereas Gpt2 expression is more restricted, mainly in liver, muscle, brain, and white adipose tissue. It has been reported that hepatic ALT2 protein is approximately four times higher in male rats than in female rats.