After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Human MICB Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human MICB Protein Product Information
Synonym:DAAP-210H10.1, PERB11.2, MICB
Protein Construction:A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was expressed with a C-terminal polyhistidine tag.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human MICB Protein QC Testing
Purity:> 98 % as determined by SDS-PAGE
Bio-Activity:Immobilized human MICB-His (Cat:10759-H08H) at 10 μg/ml (100 μl/well) can bind S4-Fc3L3-NKG2D (Cat:10575-H01S), The EC50 of S4-Fc3L3-NKG2D (Cat:10575-H01S) is 0.52-1.2 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Ala 23
Molecule Mass:The recombinant human MICB consists of 287 amino acids and has a predicted molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICB is approximately 45-50 kDa due to glycosylation.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human MICB Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other MICB Recombinant Protein Products
MICB  Background

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

Human MICB  References
  • Bauer, S. et al., 1999, Science. 285:727-729.
  • Braud, V.M. et al., 1999, Curr. Opin. Immunol. 11: 100-108.
  • Groh, V. et al., 2001, Nature Immunol. 2: 255-260.
  • Stephens, H., 2001, Trends Immunol. 22: 378-385.
  • Borrego, F. et al., 2002, Mol. Immunol. 38: 637–660.
  • Groh, V. et al., 2002, Nature. 419:734-738.
  • Product nameProduct name
    Size / Price
    Catalog: 10759-H08H-50
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions
    • Human MICB Protein (His Tag) SDS-PAGE
    Recently Viewed Items