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Hepatitis C virus (HCV-1a) NS4A ORF mammalian expression plasmid (Codon Optimized)

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HCV HCV-NS4A cDNA Clone Product Information
Gene_bank_ref_id:NC_004102.1
RefSeq ORF Size:162bp
cDNA Description:Full length Clone DNA of Hepatitis C virus (HCV) (serotype 1a, isolate H77) NS4A.
Gene Synonym:HCV-NS4A
Species:HCV
Vector:pGEM-T Vector
Plasmid:pGEM-HCV1a-NP751925-NS4A
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence NC_004102.1 (5313..5474), corresponding to amino acid sequence NP_751925.1.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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Catalog: VG40282-G
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