|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat RRM1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81060-ACG|
|Rat RRM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81060-ACR|
|Rat RRM1 ORF mammalian expression plasmid, N-GFPSpark tag||RG81060-ANG|
|Rat RRM1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81060-ANR|
|Rat RRM1 ORF mammalian expression plasmid, C-Flag tag||RG81060-CF|
|Rat RRM1 ORF mammalian expression plasmid, C-His tag||RG81060-CH|
|Rat RRM1 ORF mammalian expression plasmid, C-Myc tag||RG81060-CM|
|Rat RRM1 ORF mammalian expression plasmid, C-HA tag||RG81060-CY|
|Rat RRM1 ORF mammalian expression plasmid, N-Flag tag||RG81060-NF|
|Rat RRM1 ORF mammalian expression plasmid, N-His tag||RG81060-NH|
|Rat RRM1 ORF mammalian expression plasmid, N-Myc tag||RG81060-NM|
|Rat RRM1 ORF mammalian expression plasmid, N-HA tag||RG81060-NY|
|Rat RRM1 Gene cDNA clone plasmid||RG81060-U|
|Rat RRM1 natural ORF mammalian expression plasmid||RG81060-UT|
|Learn more about expression Vectors|
RRM1 is a subunit of ribonucleoside-diphosphate reductase which is constituted by two subunits. Ribonucleoside-diphosphate reductase is an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. RRM1 is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. RRM1 may play a role in malignancies and disease that involve this region.