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Carbonic Anhydrase XII / CA12 Antibody, Mouse MAb

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Human CA12 Antibody Product Information
Immunogen:Recombinant Human Carbonic Anhydrase XII / CA12 Protein (Catalog#10617-H08H)
Clone ID:2D6E1
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase XII / CA12 (rh Carbonic Anhydrase XII / CA12; Catalog#10617-H08H; NP_001209.1; Met 1-Gln 291 B). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CA12 Antibody Usage Guide
Specificity:Human Carbonic Anhydrase XII / CA12
No cross-reactivity in ELISA with
Mouse Carbonic Anhydrase XII / CA12
Human Carbonic Anhydrase II / CA2
Human Carbonic Anhydrase III / CA3
Human Carbonic Anhydrase IV / CA4
Human Carbonic Anhydrase VA / CA5A
Human Carbonic Anhydrase VB / CA5B
Human Carbonic Anhydrase VIII / CA8
Human Carbonic Anhydrase X / CA10
Human Carbonic Anhydrase XIII / CA13
Human Carbonic Anhydrase XIV / CA14
Human Carbonic Anhydrase IX / CA9
Human cell lysate (293 cell line)

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect CA12-His. The detection limit for CA12-His is approximately 0.0195 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other CA12 Antibody Products
Carbonic Anhydrase XII Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I  membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

Human Carbonic Anhydrase XII References
  • Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810–11813.
  • Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601.
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 16903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
  • Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
  • Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
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